Journal: Frontiers in Cell and Developmental Biology

Article Title: Biallelic Mutations in ACACA Cause a Disruption in Lipid Homeostasis That Is Associated With Global Developmental Delay, Microcephaly, and Dysmorphic Facial Features

doi: 10.3389/fcell.2021.618492

Figure Lengend Snippet: Summary of the genetic characteristics and SDS-PAGE of ACC1 protein content and ACC1 enzyme activity. (A) The pedigree of the affected individual. The proband was represented as black solid circle and pointed out by an arrow. The square indicates male, and the circle female. (B) Sanger sequencing of the mutations. (C) The amino acid conservation among different species. (D) The functional domain of ACC1, and the mutation sites were marked. (E) Relative ACC1 protein content of lymphocytes. (F) The ACC1 enzyme activity among the proband- and age-matched control-derived lymphocytes. One-way ANOVA applied in panels (E,F) . ** p < 0.01.

Article Snippet: According to the Human Protein Atlas, ACC1 expression level in fibroblasts is almost 66.7% compared to HUVEC, however, in our data the expression level in fibroblasts and lymphocytes was even higher than HUVEC ( ).

Techniques: SDS Page, Activity Assay, Sequencing, Functional Assay, Mutagenesis, Control, Derivative Assay

Journal: Frontiers in Cell and Developmental Biology

Article Title: Biallelic Mutations in ACACA Cause a Disruption in Lipid Homeostasis That Is Associated With Global Developmental Delay, Microcephaly, and Dysmorphic Facial Features

doi: 10.3389/fcell.2021.618492

Figure Lengend Snippet: ACC1-knockdown (KD) fibroblasts and the cell motility capacity measurement. (A) The ACC1 protein content of ACC1-KD fibroblast cell lines, and (B) the ACC1 enzyme activity. The wound healing assay was carried out at different time points (0, 12, and 24 h) (C) without or (E) with palmitate (scale bar = 30 μm), and (D,F) show quantitative analysis, respectively. The Trans-well migration capacity at 100× magnification (upper) and 200× magnification (lower) (G) with or (I) without palmitate, respectively. (H,J) The quantitative analyses, respectively. All data were from three independent replicates and analyzed by mean ± SEM. Independent t -test in panels (A,B,D,F,H,J) . * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: According to the Human Protein Atlas, ACC1 expression level in fibroblasts is almost 66.7% compared to HUVEC, however, in our data the expression level in fibroblasts and lymphocytes was even higher than HUVEC ( ).

Techniques: Knockdown, Activity Assay, Wound Healing Assay, Migration

Journal: Leukemia

Article Title: The protein deacetylase HDAC10 controls DNA replication in malignant lymphoid cells

doi: 10.1038/s41375-025-02612-8

Figure Lengend Snippet: A Comparison of HDAC10 mRNA expression levels in tumor cell lines, RNA-sequencing data are from 93 leukemia and 76 lymphoma cell lines; database: Cell line - HDAC10 - The Human Protein Atlas . B Chemical structure of PZ48. C The indicated cell types were treated with 15 µM of PZ48 for 24 h ( n = 3, mean+SD; two-way ANOVA; **** p ≤ 0.0001). Flow cytometry was used to detect Annexin-V/FITC and PI (-, untreated; +, treated). D Immunoblot was done to reveal the expression of HDAC10 in cultured ALL cells; HSP90, loading control. E Graphs indicate the expression levels of HDAC10 mRNA in 1817 ALL cells and 1858 AML cells from patients; GEXP2 log2, logarithmic gene expression; database: http://hemap.uta.fi/ . F PDX-ALLs were treated with increasing doses (2, 5, 10 and 12 µM; -, control DMSO) of PZ48 for 72 h ( n = 3, mean+SD; two-way ANOVA; * p ≤ 0.1, **** p ≤ 0.0001). Flow cytometry was used to detect Annexin-V/FITC and PI. G Immunoblot shows the levels of HDAC10 in primary ALL cells; HSP90, loading control.

Article Snippet: The Human Protein Atlas database [ ] illustrates that of various human tumor cell lines analyzed, leukemia and lymphoma cells have the highest HDAC10 mRNA expression levels ( Fig. ) .

Techniques: Comparison, Expressing, RNA Sequencing, Flow Cytometry, Western Blot, Cell Culture, Control, Gene Expression

Journal: OncoTargets and therapy

Article Title: CHFR promotes the migration of human gastric cancer cells by inducing epithelial-to-mesenchymal transition in a HDAC1-dependent manner

doi: 10.2147/OTT.S191016

Figure Lengend Snippet: CHFR expression and the association between its levels and overall survival in gastric cancer patients. Notes: ( A ) Correlation between expression of CHFR and overall survival of gastric cancer patients. Data were obtained from the Kaplan–Meier plotter dataset. ( B ) CHFR expression was compared in cancerous tissues ( C ) and paracancerous tissues (P) using IHC assay with anti-human CHFR monoclonal antibodies. Brown color indicates CHFR protein levels with counterstaining by hematoxylin in blue. Representative results are shown. ( C ) Representative images of gastric cancer tissues with different positive expression of CHFR are shown. Abbreviation: IHC, immunohistochemistry.

Article Snippet: The CHFR protein expression level is relatively abundant in human gastric cancer specimens according to The Human Protein Atlas dataset.

Techniques: Expressing, Bioprocessing, Immunohistochemistry